A Mendelian Mutation Affecting Mating-Type Determination Also Affects Developmental Genomic Rearrangements in Paramecium tetraurelita

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A Mendelian mutation affecting mating-type determination also affects developmental genomic rearrangements in Paramecium tetraurelia.

In Paramecium tetraurelia, mating type is determined during the differentiation of the somatic macronucleus from a zygotic nucleus genetically competent for both types, O and E. Determination of the developing macronucleus is controlled by the parental macronucleus through an unknown mechanism resulting in the maternal inheritance of mating types. The pleiotropic mutation mtFE affects macronucl...

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Non-Mendelian Mutation in Paramecium tetraurelia

The Paramecium tetraurelia mutant called d48 has a complete copy of the A surface protein gene in its micronuclei, but lacks the A gene in the macronucleus. Previous experiments have shown that microinjection of a plasmid containing the entire A gene or a large portion of the gene into the macronucleus of d48 rescued the cell line after formation of a new macronucleus (autogamy). Here we show t...

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Determination and inheritance of mating type in Paramecium caudatum.

M A T I N G type determination and inheritance in Paramecium caudatum has been reported only for syngen 12 (HIWATASHI 1958). Inheritance was usually synclonal, i.e. the two clones derived from the two members of a conjugating pair are identical in mating type expression, indicating direct genic control of mating type. However the breeding analysis was incomplete and some of the observations, as...

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RNA-mediated programming of developmental genome rearrangements in Paramecium tetraurelia.

The germ line genome of ciliates is extensively rearranged during development of the somatic macronucleus. Numerous sequences are eliminated, while others are amplified to a high ploidy level. In the Paramecium aurelia group of species, transformation of the maternal macronucleus with transgenes at high copy numbers can induce the deletion of homologous genes in sexual progeny, when a new macro...

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Genetic Analysis of Mating Type Differentiation in PARAMECIUM TETRAURELIA. II. Role of the Micronuclei in Mating-Type Determination.

The two complementary mating types, O and E, of Paramecium tetraurelia are normally inherited cytoplasmically. This property has generally been interpreted to indicate the presence of cytoplasmic factors that determine macronuclear differentiation towards O or E. In these macronuclear-cytoplasmic interactions, the micronuclei were held to be unbiased and the determination to be established in t...

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ژورنال

عنوان ژورنال: Genetics

سال: 1996

ISSN: 1943-2631

DOI: 10.1093/genetics/143.1.191